Fig 2: Surface marker analysis of captured cells and porcine monocyte. The captured cells were isolated from the 3 h transplantation P-graft. a, Expression levels of CD31, CD34, CD105 and Flk-1 in CC-3H were indicated. b, Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the captured cells were plotted. c, Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the porcine monocyte were plotted. The MoN and MoP populations were expressed CD14Low/CD16Low/CD163+ and CD14 + /CD16 + /CD163 + , respectively.

Fig 3: Outgrowth capacity and surface marker analysis of captured cells a, Outgrowth capacity was evaluated on the cell culture plate. The cells were grown from the tissue after 3 days. b,c, Two-type cells were isolated from the single colony cultivation. The cells were classified into (b) filopodia-shaped (CC-3D1) and (c) spindle-shaped (CC-3D2) cells based on the difference in morphology. These cells were expressed the surface maker of CD16/CD14. d,e, Surface marker expression of CD31, CD34, CD105, and Flk-1 on (d) CC-3D1 and (e) CC-3D2 were indicated. f, Di-ac-LDL uptake of the mixture of CC-3D1 and CC-3D2 was compared to the fibroblast and endothelial cells on CLSM observation.

Fig 4: Blood response to the luminal surface of the P-graft and Un-graft. a, Gross images and SEM observation of the luminal surface of the P-graft and Un-graft. Blood was circulated for 1 h by a cardiopulmonary pump system. The red arrowhead indicates clot deposition. b, SEM images of the luminal surface of the P-graft after transplantation for 3 h, 1 day, and 3 days. c, Cross-section images of the P-graft after transplantation for 1 day. The sections were stained with HE, anti-CD31, anti-CD34, anti-CD105, and anti-Flk-1 antibodies. Asterisks indicate the luminal side. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)